Makini Auctioneers evicted Uchumi supermarkets - Mombasa Avenue branch, forcing its immediate closure.
The auctioneers removed goods from the premises after they secured court orders allowing them to evict the retailer on Tuesday.
Casual workers hired by the auctioneer removed goods inside the structure over over unpaid rent arrears allegedly amounting to Sh12 million.
Uchumi announced that its half year losses had widened by 63 per cent to Sh895 million in February this year, weeks after exiting Sarit Centre in Nairobi.
The closed branch in Mombasa was the only surviving outlet of the cash-strapped retailer in the coastal town.